Journal: Physiological reports

Article Title: AKT2 is the predominant AKT isoform expressed in human skeletal muscle.

doi: 10.14814/phy2.13652

Figure Lengend Snippet: Figure 1. RNA transcript and protein expression of AKT isoforms in human skeletal muscle. Real-time PCR was performed on cDNA derived from human vastus lateralis using AKT isoform-specific primers/probes. Expression levels are normalized to expression of AKT1 which was set to 1.0. (means SEMs; n = 4; ***, P < 0.001 versus AKT1 and AKT3). (B) Western immunoblotting was performed on synthetic peptides of full- length human AKT1, AKT2, or AKT3 using the indicated antibodies. (C) Immunoprecipitations were performed using either rabbit or mouse pan AKT antibodies followed by western blotting with isoform-specific antibodies as indicated. Twenty-five micrograms of whole cell lysate (“WCL”) were also subjected to SDS-PAGE. Control immunoprecipitations were performed using the relevant species-specific IgG instead of antibody. Molecular mass in kDa is shown on the left of each blot. “HC;” heavy chain; LC, light chain.

Article Snippet: Antibodies for AKT1 (C73H10; #2938), AKT2 (D6G4; #3063), AKT3 (E2B6R; #14293), AKT (Pan) (C67E7) (rabbit monoclonal; #4691), AKT (Pan) (40D4) (mouse monoclonal; #2920), and Protein A HRP (#12291S), were purchased from Cell Signaling Technologies (Danvers, MA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Derivative Assay, Western Blot, SDS Page, Control

Journal: Metabolites

Article Title: Sucralose Targets the Insulin Signaling Pathway in the SH-SY5Y Neuroblastoma Cell Line

doi: 10.3390/metabo13070817

Figure Lengend Snippet: List of primary and secondary antibodies.

Article Snippet: Anti-protein kinase B (AKT) , IgG, monoclonal , Mouse , Cell Signaling, Danvers, MA, USA , 2920S , 1:1000.

Techniques: Labeling

Journal: Clinical colorectal cancer

Article Title: Ganetespib, a Novel Hsp90 Inhibitor in Patients With KRAS Mutated and Wild Type, Refractory Metastatic Colorectal Cancer

doi: 10.1016/j.clcc.2014.09.001

Figure Lengend Snippet: Immunohistochemistry (IHC) Analyses of p70s6, pERK, pAKT, Cyclin D1, VEGF-r2, HIF-1A, and Heat Shock Protein (Hsp) 70 Expression 24 to 48 Hours Before (PRE) and After (POST) Treatment With Ganetespib. Of the 17 Evaluable Patients, 13 Had Adequate Tissue Sampling For IHC Analyses. Two to ≥ 3+ and > 5% Staining Was Considered Positive.

Article Snippet: Correlative Laboratory Analyses of Tumor Tissue Samples Immunohistochemical Analyses Immunohistochemistry (IHC) was performed using formalin-fixed, paraffin-embedded tumor tissue to quantitate the expression of phospho extracellular signal-regulated kinases (pErk) (XP rabbit monoclonal antibody [mAb] from Cell Signaling Technology, Inc), Cyclin D1 (rabbit mAb from Lab Vision Corp), phospho protein kinase B (pAkt) (XP rabbit mAb from Cell Signaling), hypoxia inducible factors (HIF)-1a (mouse mAb from Novus Biologicals), VEGFr2 (rabbit mAb from Cell Signaling), p70S6 (rabbit mAb from Cell Signaling), and Hsp70 (rabbit polyAb from Cell Signaling) were determined before and after treatment with drug.

Techniques: Immunohistochemistry, Expressing, Sampling, Staining